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In another embodiment, the present invention is directed toward a modified TetR(A) protein comprising an amino acid substitution at a position selected from the group consisting of positions 59, 70, 71, 91, 95, 96, 99, 100, 101, 103, 114, 127, 158, 159, 160, 179, 193, 197, and 201 of the TetR(A) protein as depicted in SEQ ID NO: 34, wherein said modified TetR(A) protein binds a TetR(A) operator sequence with greater affinity in the presence of tetracycline than in the absence of tetracycline; wherein: the amino acid substitution at position 59 is asparagine; the amino acid substitution at position 70 is valine; the amino acid substitution at position 71 is valine; the amino acid substitution at position 91 is glutamine; the amino acid substitution at position 95 is selected from the group consisting of glycine and glutamic acid; the amino acid substitution at position 95 is glycine; the amino acid substitution at position 95 is glutamic acid; the amino acid substitution at position 96 is arginine; the amino acid substitution at position 96 is glutamic acid; the amino acid substitution at position 99 is glutamic acid; the amino acid substitution at position 100 is alanine; the amino acid substitution at position 101 is histidine; the amino acid substitution at position 103 is serine; the amino acid substitution at position 114 is valine; the amino acid substitution at position 127 is selected from the group consisting of arginine, lysine, and histidine; the amino acid substitution at position 158 is cysteine; the amino acid substitution at position 159 is leucine; the amino acid substitution at position 160 is valine; the amino acid substitution at position 179 is valine; the amino acid substitution at position 193 is glycine; the amino acid substitution at position 197 is tryptophan; and the amino acid substitution at position 201 is histidine.

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2019: Recalculating

Last year was tough for me. Health wise, I struggled quite a bit. The physical pain, unfortunately, impacted my emotional health and stamina. I could barely motivate myself to get things done. And so, in this New Year, I am trying to find that motivation, that zeal for creativity again. Many people pray for a

Grateful for a Growing Family!

It’s November 1st…Happy All Saints Day!  I love this Solemnity and the time to recall all the wonderful saints who have gone before us.  While I think of some of my favorites – Saints Dominic & Catherine of Siena, Saint Maximilian Kolbe, our Blessed Mother Mary – I also think of the countless saints whose

Always be Ready

This past Sunday, our parish held its annual Parish Picnic at a beautiful county park.  We celebrated Mass under the trees with a soft late summer breeze rustling through the trees (and knocking down plenty of acorns!)  Every family brings a dish to pass, and a crew of men spent all night Saturday with grills

Thoughts on Corpus Christi

************This is the reflection I shared this weekend with my Lay Dominican group, the Mary Magdalene Chapter, which meets at St Thomas Aquinas Church on the campus of Purdue University.************** When I realized that my assigned month for doing the reflection would fall on the weekend of Corpus Christi I was so excited. I thought

Lent Begins Again

It’s late on Ash Wednesday.  My mind is racing far too much for this time of night, but that’s not unusual.  I have always been a night owl.  Before I sit down for Night Prayer (Compline) I decided I needed to write just a bit. I have quite the Lenten to-do list this year, so

#FridayFavorites: Tea Time!

I’m doing something a bit different today, and as the hashtag indicates, I hope to continue this on a regular basis.  I’ll share one of my favorite things, and see where my thoughts wander.  These probably won’t be very serious thoughts, but that can be a good thing! Earlier this month, I discovered that January

#WorthRevisit: Why We March

It’s Wednesday, and that means it is time for another #WorthRevisit post.  This weekend is the March for Life in Chicago, but Bruce and I won’t be attending.  I think I will start scheduling some tweets and contacting my friends at Students for Life Illinois, just to be there in spirit!  This post is from

He Makes All Things New

How many people take five days to write a New Year’s post?  At least one, apparently.  It has been bitterly cold here in central Illinois all week, along with most of the country.  I have been hibernating in my home office, completing 2017 Christmas cards (it IS still Christmas!) and prepping for a 12th Day